Chèvre anti-Boeuf (Vache) IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN457566

Aperçu rapide pour Chèvre anti-Boeuf (Vache) IgG (Heavy & Light Chain) Anticorps (ABIN457566)

Antigène: IgG

Clonalité: Polyclonal

Application: Immunocytochemistry (ICC), Immunodiffusion (ID), Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Boeuf (Vache)

Hôte: Chèvre

Specificité: The reactivity of the antiserum is directed to the Fc and Fab subunits of both subclasses, IgG1 and IgG2. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against bovine serum a single characteristic precipitin line with a spur is usually obtained.

Attributs du produit: Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:128 when tested against normal bovine serum in agar block titration.

Purification: Delipidated, heat inactivated lyophilized stable whole serum
No adsorption required.

Immunogène: Purified normal IgG isolated from pooled bovine serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Information d'application

Indications d'application: In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal bovine serum, to prepare an immunoadsorbent for the production of Ig-free bovine serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Agent conservateur: Without preservative

Conseil sur la manipulation: Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same da y. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Stock: 4 °C/-20 °C

Stockage commentaire: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Détail du antigène

Antigène: IgG

Classe de substances: Antibody